Journal:
Article Title: ProNGF Induces p75-Mediated Death of Oligodendrocytes following Spinal Cord Injury
doi:
Figure Lengend Snippet: (A) High-molecular weight NGF is predominantly induced after spinal cord injury. 30 μg of extracts were analyzed at each time point in Western analyses for NGF (Chemicon), BDNF (Promega), and NT3 (Promega). The extracts were prepared from rostral 5 mm (rats) or 3 mm (mice) blocks from the lesion center, but the pattern of NGF expression did not vary whether the sample was taken caudally or from the lesion center. The arrow indicates the position of recombinant BDNF and NT3, which were used at 250 ng as a control for antibody specificity. The star points to purified NGF being weakly recognized by anti-BDNF antibody.
Article Snippet: 350 μg of lysates was immunoprecipitated with proNGF antibody, and probed with anti-mature-NGF antibody (Chemicon).
Techniques: Molecular Weight, Western Blot, Expressing, Recombinant, Purification